Assay procedure summary

1. Prepare all reagents, samples, and standards;
2. Add 100µL standard or sample to each well. Incubate for 1 hour at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate for 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate for 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate for 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.  ELISA Kits are available in the following species:

• Pan-species

• Multi-species

• Human

• Simian

• Mouse

• Rat

• Canine

• Porcine

• Bovine

• Rabbit 

• Chicken

• Sheep
  

Please enquire about species-specific products and we can help find the right match for you.